Neutrophilic- Leukocyte Alkaline Phosphatase (NAP) Stain
Intended Use:
This kit is for staining bone marrow cell and bloodcell smear.
Principle:
Neutrophilic-Leukocyte Alkaline Phosphatase stain is based on diazotization-coupling principle. At pH9.2~9.8, Alkaline Phosphatase hydrolyzes Naphthol AS-BI Phosphoric Acid into α-naphthol. The stable diazonium salt and naphthol shall form insoluble colored precipitate in cytoplasm.
Specifications:
| Contents | 5Tests/Kit | 20Tests/Kit | 100Tests/Kit | Components |
| Fixative (solution A) | 1vial×5ml | 1vial×20ml | 1vial×100ml | Sodium Citrate |
|
FBB -Alkaline (solution B) |
1vial×0.3ml | 1vial×1.1ml | 1vial×5.1ml |
Fast Blue BB Base |
|
Sodium Nitrite (solution C) |
1vial×0.3ml | 1vial×1.1ml | 1vial×5.1ml | Sodium Nitrite |
|
Naphthol AS-Bl Alkaline (solution D) |
1vial×0.3ml | 1vial×1.1ml | 1vial×5.1ml | Naphthol AS-Bl |
|
Neutral Red (solution E) |
1vial×5ml | 1vial×20ml | 1vial×100ml | Neutral Red |
Methods:
Working solution Preparation (for 1 test only)
Apparatus required：disposable tube, micropipette, disposable tips, dropper;
Instruction：Mix 25µl solution B with 25µl C; wait for 2 minutes. Add distilled water 2ml into 25µl solution D. Mix thoroughly.
Expected Results:
NΑP positive granules are blue.
